Carbon Dioxide Before implementing any euthanasia procedure, be sure to read Euthanasia Process. At the present time, many in the wildlife control industry consider euthanasia by carbon dioxide (bottled gas only) induced narcosis to be the most user-friendly of the AVMA suggested methods. This is the method commonly used for the euthanasia of laboratory research animals. Using this method requires a chamber in which the heavier-than-air carbon dioxide replaces available oxygen in a container. The opportunity to euthanize the animal without injection, handling, or transfer is a definite advantage of this method. Carbon dioxide is readily available at welding supply centers, is relatively safe to the wildlife technician, and will suppress an animal’s ability to experience pain prior to death. To euthanize by CO2 you will need the following equipment: A euthanasia chamber (can be built or purchased). Your chamber should be top loading and large enough to hold your largest cage. Some wildlife controllers have different size chambers for different size traps to save gas. Chambers can be as simple as a wooden box or a plastic trashcan or barrel. Seal the bottom and sides with glue and/or caulk so that they are air tight. The lid, however, should not be air tight. Drill a hole for the CO2 delivery tube in the side of the box around 1 inch from the bottom. For size requirements see Step 2. CO2 is heavier than air. As the gas enters the chamber, the heavier, CO2 displaces the lighter, room-air out the top of the box. This is why the lid should fit securely but not be air tight. Visit a welding supply company or bottled gas supplier. They can provide you with the CO2 tank, gas regulator, flow meters, and tubing. Ask the welding supply company about all relevant laws and regulations concerning the handling, storage, and transportation of pressurized tanks. Improper handling of tanks can result in serious injuries and property damage. Here is how to calculate the flow rate for your particular chamber. Measure the chamber’s internal length, width, and height in inches. Multiply those 3 numbers (length x height x width) to determine the chamber’s volume in cubic inches. For example, a 13 x 13 x 33-inch tank = 5,577 cu inches in volume. Divide by 61 to convert the volume to liters (5,577 divided by 61 = 91.39661 liters or 9.14). Then multiply 91.4 by .20 because we only want 20% of the tank to fill per minute. This comes to 18.28 liters. Therefore, you should set your gravity flow meter to a little more than 18 liters per minute and leave it on to fill the tank completely in 5 minutes. After the chamber has filled, reduce the rate of flow to 3 to 5 liters per minute (lpm) to save CO2. Do not turn the flow off as you will need to maintain positive pressure to prevent fresh air from re-entering the chamber. Some animals, like squirrels and adult raccoons, will expire quickly. Others, like skunks, infant raccoons, and woodchucks, can take 30 minutes or more to die. In all circumstances, it is imperative that the animal’s death be confirmed.